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Genetic diversity and connectivity of Flaccisagitta enflata (Chaetognatha: Sagittidae) in the tropical Atlantic ocean (northeastern Brazil) ArchiMer
Melo, Danielle C. M.; Lira, Simone M. A.; Moreira, Ana Paula B.; Freitas, Lucas; Lima, Camilla A. D.; Thompson, Fabiano; Bertrand, Arnaud; Silva, Alex C.; Neumann-leitão, Sigrid.
The phylogeography of the holoplanktonic chaetognath Flaccisagitta enflata was investigated in the Tropical Western Atlantic (TWA). Considering the cosmopolitan range of this species and the fact that its entire life cycle is planktonic, the central hypothesis of this study is that F. enflata exhibits connectivity due to its high dispersal capacity, forming a panmictic population among the study sites. The evaluated areas included neritic (Port of Recife–PR, and Tamandaré - TA) and oceanic (Fernando de Noronha Archipelago—FN, Rocas Atoll—RA, Guará seamount—GS and Saint Peter and Saint Paul’s Archipelago—SPSPA) locations of the Brazilian Blue Amazon. We used COI gene sequences as molecular marker. Partial sequences (425 bp) were obtained for 116 specimens...
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Ano: 2020 URL: https://archimer.ifremer.fr/doc/00630/74173/73744.pdf
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Transcriptomic analysis of clam extra pallial fluids reveals immunity and cytoskeleton alterations in the first week of Brown Ring Disease development ArchiMer
Rahmani, Alexandra; Corre, Erwan; Richard, Gaëlle; Bidault, Adeline; Lambert, Christophe; Oliveira, Louisi; Thompson, Cristiane; Thompson, Fabiano; Pichereau, Vianney; Paillard, Christine.
The Brown Ring Disease is an infection caused by the bacterium Vibrio tapetis on the Manila clam Ruditapes philippinarum. The process of infection, in the extrapallial fluids (EPFs) of clams, involves alteration of immune functions, in particular on hemocytes which are the cells responsible of phagocytosis. Disorganization of the actin-cytoskeleton in infected clams is a part of what leads to this alteration. This study is the first transcriptomic approach based on collection of extrapallial fluids on living animals experimentally infected by V. tapetis. We performed differential gene expression analysis of EPFs in two experimental treatments (healthy-against infected-clams by V. tapetis), and showed the deregulation of 135 genes. In infected clams, a...
Tipo: Text Palavras-chave: Brown ring disease; V. tapetis; R. philippinarum; Hemocytes; Actin cytoskeleton; Beta-Thymosin; Coactosin; Resting cells.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00510/62131/66362.pdf
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